The characteristics of crude enzyme produced from Trichoderma viride(O₂-1) which isolated from half spoiled Locust acacia wood (Robinia Pseudacacia Linne) were examined in this paper.
The results obtained were summarized as follows:
1) As a results of enzymatic action on several cellulose substrate it was found that there were some sorts of cellulase in crude enzyme.
2) The activity of C.M.C. ase and β-glucosidase were decreased in accordance with increasing concentration of substrate, and filter paper saccharifying enzyme relatively increased in accordance with increasing concentration of substrate and enzyme in couse of time.
3) The optimal pH of each enzyme was 5.0 and the range of stability on pH generally from 3 to 6.
4) In disintegrating activity on filter paper, in decomposing activity on C.M.C. and p-nitrophenyl-β-D-glucoside, and in saccharifying activity on filter paper, the optimal temperatures were 50℃, 60℃, and 65℃.
5) The order of stability on temperature was as follow; saccharifying activity on filter paper decomposing activity on C.M.C. disintegrating activity on filter paper$gt;decomposing activity on p-nitrophenyl-β-D-glucoside.
6) The activity of the enzymes was inhibited with mercuric and silver ion, and activated with potassium.
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